REDUCTION of BACTERIAL TRANSLOCATION and INTESTINAL STRUCTURAL ALTERATIONS by HEPARIN in a MURINE BURN INJURY MODEL11
XY. Zapata-Sirent RL, Hansbrough JF, Greenleaf GE, Grayson LS, and Wolf P. Reduction of bacterial translocation and intestinal structural alterations by hepain in a murine burn injury model. The J of Trauma. 1994;36(1):1-6.
Burn injury produces acute gastrointestinal (Gl) derangements that may predispose the burn victim to bacterial translocation (BT). We studied the effects heparin on gastrointestinal (GI) anatomic alterations and BT after 25% and 32% total body surface area (TBSA), full-thickness murine burn injuries. Heparin (100 U/kg) was administered with 1 ml of normal saline (NS) resuscitation solution immediately postburn, and 4 hours and 18 hours postburn in volumes 0.5 mL NS. Mice with 25% TBSA burns treated with heparin maintained small intestine weight, measured at 24 hours postburn, and ileal mucosal height was preserved, whereas burned, untreated mice lost organ weight and mucosal height. Bacterial translocaion was decreased in mice with 25% TBSA injuries treated with heparin (35.0% vs. 10.7%, p <0.025). After 32% TBSA burn injuries, BT was also decreased in heparin-treated animals (64.3% vs. 31.6%; p <0.025). Analysis of mixed venous blood gases showed that heparin did not affect the severe metabolic acidosis that follows burn injury in this animal models, indicating that general tissue perfusion was not improve. Heparin administered in the acute postburn period ameliorates GI structural and functional damage in this murine burn model and decreases BT.